The country recorded 506 new Covid-19 cases on Friday. The previous daily record was 373 cases on March 27.
The health ministry noted in a tweet that Friday's record number corresponds with an increased number of tests carried out that day, targeting areas where infections have already been detected.
On Friday, 8,532 tests were carried out. This compares to 5,695 on Thursday and 7,312 on Wednesday.
A total of 411 Covid-19 deaths have been recorded in the Czech Republic, according to the health ministry.